Human Umbilical Cord Blood Serum in Culture Medium on Oocyte MaturationIn Vitro
نویسندگان
چکیده
منابع مشابه
Unrestricted somatic stem cells from human umbilical cord blood grow in serum-free medium as spheres
BACKGROUND Human umbilical cord blood-derived unrestricted somatic stem cells (USSCs), which are capable of multilineage differentiation, are currently under investigation for a number of therapeutic applications. A major obstacle to their clinical use is the fact that in vitro expansion is still dependent upon fetal calf serum, which could be a source of pathogens. In this study, we investigat...
متن کاملEndothelial progenitor cell culture and differentiation in vitro: a methodological comparison using human umbilical cord blood.
OBJECTIVE Endothelial progenitor cells (EPC) can contribute to vascular repair and targeted tumour therapy. Little is known about generating EPC from human umbilical cord blood. We therefore compared methods for purification of EPC from human umbilical cord blood. METHODS Mononuclear cells were isolated from human umbilical cord blood by density gradient centrifugation and used either unselec...
متن کاملxeno-free culture condition for human bone marrow and umbilical cord matrix-derived mesenchymal stem/stromal cells using human umbilical cord blood serum
abstractbackground: fetal bovine serum (fbs) is widely used in cell culture laboratories,risk of zoonotic infections and allergic side effects create obstacles for its use inclinical trials. therefore, an alternative supplement with proper inherent growthpromotingactivities is demanded.objective: to find fbs substitute, we tested human umbilical cord blood serum(hucs) for proliferation of human...
متن کاملXeno-free culture condition for human bone marrow and umbilical cord matrix-derived mesenchymal stem/stromal cells using human umbilical cord blood serum
BACKGROUND Fetal bovine serum (FBS) is widely used in cell culture laboratories, risk of zoonotic infections and allergic side effects create obstacles for its use in clinical trials. Therefore, an alternative supplement with proper inherent growth-promoting activities is demanded. OBJECTIVE To find FBS substitute, we tested human umbilical cord blood serum (hUCS) for proliferation of human u...
متن کاملComparison of Maternal Serum and Umbilical Cord Blood Leptin Level in IUGR Neonates
Background: Gestational weight gain is an impressive factor in the fetal outcome. Intrauterine growth restriction (IUGR) is one of the most important problems during fetal period that may lead to many perinatal and long-term complications and growing neonatal morbidities and mortalities. The aim of the study was to ascertain the relationship between umbilical cord blood leptin concentration and...
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ژورنال
عنوان ژورنال: Archives of Andrology
سال: 2007
ISSN: 0148-5016
DOI: 10.1080/01485010701730948